TABLE 1.

Plasmids and strains used in this study

Plasmid or strainCommentsaReference or source
Plasmids
    pSWEETB. subtilis suicide vector for integration at amyE3
    pHWattB-CDpSWEET containing the 436-bp attB-CD cloned in the BamHI siteThis work
    pHWattB-CD2pSWEET containing two copies of attB-CD in direct repeatThis work
    pHWattB-CDTCpSWEET containing attBcd(TC)This work
    pHWattB50pSWEET containing the 50-bp attB-CDThis work
    pHWattLpSWEET containing attLThis work
    pHWattRpSWEET containing attRThis work
    pHWattTnpSWEET containing attTnThis work
Strains
    C. difficile CD630Tcr Emr C. difficile strain containing Tn5397 and Tn539812
    B. subtilis CU2189Tcs recipient strain6
  • a Tc, tetracycline; Em, erythromycin.