TABLE 1.

Bacterial strains and plasmids

Strain or plasmidRelevant phenotype and genotypeSource or reference
E. coli strains
    BL21(DE3)/pLysSF ompT hsdSB (rBmB) gal dcm (DE3) carrying pLysSNovagen
    DH10BFmcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 endA1 recA1 deoR Δ(ara,leu)7697 araD139 galU galK nupG rpsL λInvitrogen
    HB101F thi-1 hsd20 (rBmB) sup E44 recA13 ara-14 leuB6 proA2 lacY1 rpsL20 (Smr) xyl-5 mtl-15
    Sφ200RifmetB strA purB Δ(agg-uidA-man) Rifr35
P. stewarti subsp. stewartiii strains
    DC283SS104 wild-type; Wts+, HR+, Nalr7
    DM064DC283 hrpY1296::Tn5 (Kanr)11
    DM733DC283 hrpY64::Tn5 hrpS1::Tn3HoHoI (Kanr Ampr)This study
    MEX1DC283 hrpS1::Tn3HoHo (Ampr)11
    MM254DC283 hrpY[D57N]25
Plasmids
    pBluescript KS and SK(+)ColE1 αlacZ (Apr)Stratagene
    pET15bColE1; T7lac; poly-His tag expression vector (Apr)Novagen
    pPL6GUSCpLAFR6 derivative carrying a promoterless uidA gene (Tcr)17
    pRK415IncP αlacZ (Tcr)16
    pDM2912pPL6GUSC with a +629 to −231 hrpSΔ(−111 to −64) PCR fragment fused to uidAThis study
    pMM46pBluescript KS(+) with a 0.7-kb HindIII hrpY PCR fragment, transcribed from Plac25
    pMM50pPL6GUSC with a +994 to −317 hrpS PCR fragment fused to uidA25
    pMM52pRK415 with the 0.7-kb BamHI-EcoRI hrpY+ fragment from pMM46, transcribed from Plac25
    pMM57pBluescript KS(+) with a 0.7-kb HindIII hrpY[D57A] spliced overlap extension-PCR fragment25
    pMM58pBluescript SK(+) with a 5.3-kb insert containing hrpL+, hrpXY+, and hrpS+25
    pMM74pRK415 with the 0.7-kb HindIII hrpY[D57A] fragment from pMM57; transcribed from PlacThis study
    pMM92pBluescript KS(+) with a 0.7-kb HindIII hrpY[D57N] SOE-PCR fragment25
    pMM118pRK415 with a 0.7-kb HindIII hrpY[D57N] fragment from pMM92This study
    pMM221pET15b with a 0.7-kb NdeI hrpY PCR fragmentThis study
    pMM222pET15b with a 0.7-kb NdeI hrpY[D57N] PCR fragmentThis study
    pMM396pPL6GUSC with a +629 to −165 hrpS PCR fragment fused to uidAThis study
    pMM393pPL6GUSC with a +629 to −90 hrpS PCR fragment fused to uidAThis study
    pMM400pPL6GUSC with a +629 to −231 hrpS PCR fragment fused to uidAThis study
    pMM403pPL6GUSC with a +629 to −51 hrpS PCR fragment fused to uidAThis study
    pMM1001pDM2945 with DR1 site from −99 to −90 deletedThis study
    pMM1002pDM2945 with DR2 site from −78 to −69 deletedThis study
    pMM1100pDM2945 with IHF site from −191 to −179 deletedThis study
    pMM1101pDM2945 with a 5-bp deletion from −144 to −140This study
    pMM1102pDM2945 with a 5-bp deletion from −139 to −135This study
    pMM1103pDM2945 with a 10-bp deletion from −144 to −135This study
    pDM2912pPL6GUS with pMM400 BamHI insert carrying a DR1-DR2 deletion from −121 to −70This study
    pDM2932pPL6GUS with pMM400 BamHI insert carrying a DR1-DR2 deletion from −99 to −69This study
    pDM2945pUC19 with the pMM400 BamHI insert carrying the +629 to −231 hrpS regionThis study
    pDM2949pPL6GUS with pMM1001 BamHI insertThis study
    pDM2953pPL6GUS with pMM1002 BamHI insertThis study
    pDM2958pPL6GUS with pMM1100 BamHI insertThis study
    pDM2960pPL6GUS with pMM1101 BamHI insertThis study
    pDM2963pPL6GUS with pMM1102 BamHI insertThis study
    pDM2965pPL6GUS with pMM1103 BamHI insertThis study