TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidGenotype or relevant characteristic(s)Reference or source
S. aureus
    RN42208325-4 r 10
    ISP7948325-4 pig-131 25
    QT1ISP794 mgrA::cat 28
    QT11ISP794 norG::cat This study
    QT12ISP794 mgrA::cat norG::cat This study
E. coli DH5αF φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 phoA hsdR17(rKmK ) supE44 λ thi-1 gyrA96 relA1 Gibco-BRL
Plasmids
    pGEM3-zf(+)2.9-kb E. coli cloning vector; Apr Promega
    pLI50Shuttle cloning vector (Apr Cmr) 23
    pCL52.2Temperature-sensitive E. coli-S. aureus shuttle vector 23
    pSK95010.5-kb plasmid carrying the attP site of phage L54a, replicon of pE194; Tcr Emr (S. aureus) 15
    pQT13pSK950-norG This study
    pQT14pSK950-abcA This study