TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionReference or source
Rhodobacter sphaeroides strains
    WS8Wild type55
    LC1flgE1::aadA4
    SP13fleQΔ::kan42
    SP18flgC1::kanThis study
    SS1flhA2::uidA-aadAThis study
    SS2flgC1::kan flhA2::uidA-aadAThis study
    SS3flgE1::aadA flaA::kanThis study
    SP19SP18 fla2+ carrying flgE2::aadAThis study
Escherichia coli strains
    LMG174Protein expression strainInvitrogen
    S17-1recA endA thi hsdR RP4-2 Tc::Mu::Tn7, Tpr Smr44
    TOP10Cloning strainInvitrogen
Plasmids
    pRKflgE2pRK415 derivative expressing flgE2This study
    pWM5Source of the uidA-aadA cassette34
    pJQ200mp18Conjugative suicide plasmid45
    pUC4KanSource of the Kanr cassettePharmacia
    pBADflgE2pBAD-His B derivative overexpressing flgE2This study
    pBAD-His BE. coli expression vectorInvitrogen
    pRK415Conjugative expression vector24
    pTZ19RE. coli cloning vector, Apr pUC derivativeFermentas