TABLE 3.

Complementation of LPS mutant strains with xcpQ wild-type genes and chimerasa

StrainMutationbSecretion with different xcpQ genes
NonexcpQaerxcpQalcxcpQF87xcpQF497
PAO25None++++++++++
PAN1xcpQ++++
PAN15xcpQ, suppressor+++++++
PAN16xcpQ, suppressor+++++++
PAN38xcpQ, core++++
PAN39xcpQ, O antigen++
  • a Secretion was analyzed on elastin plates by evaluating halo formation after 24 h of incubation and by analyzing extracellular protein profiles by SDS-PAGE. ++, secretion comparable to that of wild-type PAO25 cells; +, decreased but significant secretion; −, no significant secretion.

  • b core, mutation causing a defective LPS outer core; O antigen, mutation causing a lack of O antigen.