TABLE 3.

Susceptibilities of strains to quinolones and other agents

Antimicrobial or dyeMIC (μg/ml) for straina: Side chain partition coefficientb
ISP794QT1QT11QT12ISP794(pQT13)ISP794(pQT14)
Quinolones
    Norfloxacin0.540.5220.5
    Ciprofloxacin0.2520.25110.25
    Moxifloxacin0.060.50.060.250.250.06
    Sparfloxacin0.12510.060.50.50.125
β-Lactams
    Nafcillin0.50.25210.5644,200
    Penicillin G0.030.010.1250.060.032270
    Methicillin42168432
    Cefotaxime0.50.25210.546
Chemical compounds and dyes
    Cetrimide0.520.520.50.5
    TPP0.52240.58
    Rhodamine12120.54
    Ethidium bromide244824
    Hoechst 33342142422
  • a Strain QT1, ISP794 mgrA::cat; QT11, ISP794 norG::cat; QT12, ISP794 mgrA::cat norG::cat. pQT13, construct pSK950-norG; pQT14, construct pSK950-abcA. Strains harboring plasmids pQT13 and pQT14 were grown in the presence of tetracycline (5 μg/ml) at 30°C.

  • b The side chain partition coefficients were determined by Nikaido et al. (16) and represent the calculated octanol-water partition coefficients of the 6-substituents and the 7-substituents of the β-lactams.