TABLE 1.

Strains and plasmids used in this study

Strain or plasmidGenotype or descriptionSource or reference
E. coli strains
    TG1(F′ lacIq Tn10 mcrA Δ(mrr-hsdRMS-mcrBC) supEΔ(lac-proAB) thi hsdRΔ5 (F′ traΔ36proA+B+ZΔM15)Laboratory collection
    CC118 (λpir)Host strain for pKNG101 replication; Δ(ara-leu) araD ΔlacX74 galE galK-phoA20 thi-1 rpsE rpoB argE(Am) recA1 Rfr (λpir)Laboratory collection
P. aeruginosa strains
    PAO1Wild-type prototrophLaboratory collection
    PAO1MWild-type prototroph from L. Mac Carter's laboratory9
    PAO1DHWild-type prototroph from D. Haas's laboratory41
    PA103Cytotoxic respiratory clinical isolate; FlaLaboratory collection
    PAO1ΔfliC1PAO1 ΔfliCLaboratory collection
    PAO1ΔpscN1PAO1 ΔpscNThis sudy
    PAO10011PAO1M ΔmotABPA1460-1461)9
    PAO10021PAO1M ΔmotCDPA4954-4953)9
    PAO10031PAO1M ΔmotY9
    PAO6281PAO1DH gacA::ΩSp/Sm41
Plasmids
    pRK2013Tra+ Mob+ KmrLaboratory collection
    pKNG101Suicide vector in P. aeruginosa; SacB Str25
    pSBC22pKNG101 derivative; mutator of the pscN geneThis study
    pMMB190Broad-host-range tac promoter; AprLaboratory collection
    pSBC6exsA cloned in pMMB190 under control of tacp2
    pDN19lacΩBroad-host-range lacZ transcriptional fusion; Str45
    pMS565Broad-host-range pfliA-lacZ reporter; Str43
    pMP220Broad-host-range lacZ transcriptional fusion; TcrLaboratory collection
    pSB307Broad-host-range pS-lacZ reporter; Tcr2
    pSB302Broad-host-range pT-lacZ reporter; Tcr2
    pSB303Broad-host-range pY-lacZ reporter; Tcr2
    pSB305Broad-host-range pD-lacZ reporter; Tcr2
    pSB308Broad-host-range pG-lacZ reporter; Tcr2
    pSB313Broad-host-range pN-lacZ reporter; Tcr2
    pPP4Broad-host-range pC-lacZ reporter; Tcr2