TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionSource or reference
E. coli strain
    DH5αhsdR17 recA1Life Technologies
S. aureus strains
    RN4220Derivative of 8325-4 that accepts plasmids15
    8325-4Derived from 8325, missing three prophage23
    879R4RFRifr Fusr, presumed restriction deficient29
    COLLaboratory strain, pT18113
E. faecalis strain
    FA373JH2-2 carrying pAM3783
Plasmids
    pSK5632Replicates in S. aureus and E. coli (shuttle vector), Ampr Cmr9
    phsdRSau1hsdR from 8325-4 cloned in pSK5632This study
    pMADReplicates in E. coli (suicide vector), Ampr ermC1
    pT181Replicates in S. aureus, Tetr13
    pAM378Pheromone-responsive plasmid, replicates in enterococci, carries Tn918, Tetr3