TABLE 1.

Plasmids used in this study

PlasmidCharacteristicsSource or reference
pBlueScript II SK+ (pBSK)Cloning vector, AmprStratagene
pBSK::as-ahpC100100 bp as-ahpC cloned in pBSK; AmprThis study
pBSK::as-ahpC250250 bp as-ahpC cloned in pBSK; AmprThis study
pBSK::as-ahpCFull length as-ahpC cloned in pBSK; AmprThis study
pET-29bSix-His-tagged C-terminal overexpression vector; KmrNovagen
pET-29b::ahpCahpCHp cloned in pET-29b; KmrThis study
pDH37H. pylori/E. coli shuttle vector; Kmr25
pDH37::as-ahpC100100 bp as-ahpC cloned in pDH37; KmrThis study
pDH37::as-ahpC250250 bp as-ahpC cloned in pDH37; KmrThis study
pDH37::as-ahpCFull length as-ahpC cloned in pDH37; KmrThis study
pBC KS+Cloning vector; CmrStratagene
pHP1aphA3 cassette; Kmr30
pFDfd bacteriophage terminator cloned in pBC; CmrThis study
pRDX-K+rdxA-replacement suicide vector; Cmr; KmrThis study
pAS1563-2Full length as-ahpC driven by PureA and terminated by fd bacteriophage terminator cloned in pRDX-K+This study