TABLE 2.

Primers used to construct P. aeruginosa mutant strains

Primer nameSequencea
kynA upstream 15′-TACAAGAAAGCTGGGTGCAGGCATATCCGCTGCGGTTCCA-3′
kynA upstream 25′-GATGATGCGCTCCACCGTGGTCACCTGGGCCTGGGAGTGAGGGCAAGG-3′
kynA downstream 15′-GGCGTGATCTACCTCGACGGCAACTTCACCCCGCTTTACACCCGCTTC-3′
kynA downstream 25′-TACAAAAAAGCAGGCTTCGACCCCCGCTGTTTCGGCTACC-3′
kynB upstream 15′-TACAAGAAAGCTGGGTGCGACGCTGGATGATCAGGTTGCG-3′
kynB upstream 25′-CACGTCGTCGAGGACCACGCCTTCCCATTCCTGCTGGAACGGCGTGTC-3′
kynB downstream 15′-GACACGCCGTTCCAGCAGGAATGGGAAGGCGTGGTCCTCGACGACGTG-3′
kynB downstream 25′-TACAAAAAAGCAGGCTTTCGATGAACAGATCGGCGAGGGC-3′
kynU upstream 15′-TACAAGAAAGCTGGGTTGCAGGTGACGGAGAATGCGCAGA-3′
kynU upstream 25′-GAAGCGGGTGTAAAGCGGGGTGAAGTTGCCGTCGAGGTAGATCACGCC-3′
kynU downstream 15′-GGCGTGATCTACCTCGACGGCAACTTCACCCCGCTTTACACCCGCTTC-3′
kynU downstream 25′-TACAAAAAAGCAGGCTGCCAGGGTCAGCAGGTAGAACACC-3′
phnA upstream 15′-TACAAAAAAGCAGGCTCGCTGGTTGAAGGAGGGATCAGCC-3′
phnA upstream 25′-CAGCGCCTTGTTGCGGGTTTCCTCGCAGTCGAACAGCATCCGGTTGGC-3′
phnA downstream 15′-GCCAACCGGATGCTGTTCGACTGCGAGGAAACCCGCAACAAGGCGCTG-3′
phnA downstream 25′-TACAAGAAAGCTGGGTGACATGCTGCGTCTGGTGGAAGCC-3′
  • a att sequences are underlined.