TABLE 1.

Bacterial strains and plasmids

Strain or plasmidRelevant genotype or phenotypeReference or source
Strains
    E. coli
        DH5αF′/endA1 hsdR17 supE44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF)U169 deoR [φ80dlacΔ(lacZ)M15recA1] 51
        One Shot ccdB survival T1 phage-resistant cellsInvitrogen
    P. aeruginosa
        PAO1Wild type 18
        PA14Wild type 40
        PJF-KA1 kynA deletion mutant derived from strain PAO1This study
        PJF-KB1 kynB deletion mutant derived from strain PAO1This study
        PJF-KU1 kynU deletion mutant derived from strain PAO1This study
        PJF-PHNA1 phnA deletion mutant derived from strain PAO1This study
    S. aureus ATCC 25923Clinical isolate; laboratory test strainATCC
Plasmids
    pEXGWApGateway cloning vector adapted for P. aeruginosa M. Wolfgang
    pΔkynAsuc kynA deletion suicide vectorThis study
    pΔkynBsuc2 kynB deletion suicide vectorThis study
    pΔkynUsuc kynU deletion suicide vectorThis study
    pΔphnAsuc2 phnA deletion suicide vectorThis study
    pLP170 lacZ transcriptional fusion vector 39
    pMTP121Minimum tiling pathway cosmid 121 which contains kynB; Tetr 20
    pMTP153Minimum tiling pathway cosmid 153 which contains kynA; Tetr 20
    pJF01 kynB′-lacZ transcriptional fusionThis study
    pJF03 kynA′-lacZ transcriptional fusionThis study
    pUCP22 E. coli/P. aeruginosa cloning vector 49
    pJF100 phnAB complementation plasmidThis study