TABLE 1.

Bacterial strains and plasmids

Strain or plasmidRelevant characteristic(s)Reference or source
Strains
    S. flexneri 2a 2457T derivatives
        BS184 mxiC::mudI1734 Kmr 30
    E. coli K-12 derivatives
        BL21(DE3)F dcm ompT hsdS galcIts857 ind1 sam7 nin5 lacUV5-T7gene1) 53
        CJD1634BE1414/pACYC184 4
        CJD1635BE1414/pAF201 4
        XL1-Blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacI q ZΔM15 Tn10 (Tcr)]Stratagene
Plasmids
    pACYC184Cloning vector; p15A replicon; Cmr Tcr New England Biolabs
    pBAD33VirB virB gene subcloned behind arabinose-inducible PBAD promoter; Apr 4
    pBluescriptCloning vectorStratagene
    pET22bH-NS hns gene in pET-22bThis work
    pET22bVirB virB gene in pET-22b 31
    pKK232.8Promoter cloning vector 9
    pZep08GFP reporter plasmid 20
    pZep-proicsB-2 icsB promoter cloned into pZEP08This work
    pZep-proicsB-41-bp substitution in box 1This work
    pZep-proicsB-5First 3 bp in box 1 replacedThis work
    pZep-proicsB-6Second 3 bp in box 1 replacedThis work
    pZep-proicsB-7Box 1 sequence invertedThis work
    pZep-proicsB-8All box 1 bases replacedThis work
    pZep-proicsB-9Box 2 sequence invertedThis work
    pZep-proicsB-10All box 2 bases replacedThis work
    pZep-proicsB-113′ end-flanking CCC replacedThis work