TABLE 2.

Oligonucleotides used in this study

PrimerSequence (5′ to 3′)a
AHPCA2FTTCCCGGGATGTTAGTTACAAAACTTGC
AHPCA2RGAGAATTCAGAAAATTCCATTAAGCTT
AHPCAFTTCCCGGGAATAACGATGAAACAAGA
AHPCARACGAATTCATCGTAACTCCTTAAGTG
AAHPCFBHICAGGGATCCAATAACGATGAAACAAGA
AAHPC100BPECAGGAATTCCATGGTGAAGTTTGCCCAGCA
AAHPC250BPECAGGAATTCGAAGAAGCGATCGCTTTGAG
AHPCFBamHIGCGGATCCGATGTTAGTTACAAAACTTGCCCCA
AHPCRXhoICCGCTCGAGAAGCTTAATGGAAT
RDXAIFSacIGTAGAGCTCGCATTCGTGGGATGAGCTA
RDXAIRXbaIGCATCTAGACTTGCAAGAATGGCGCTCG
RDXAIIFXhoICGACTCGAGGTGGCAGAAGCGAGTCA
RDXAIIRKpnICTGGGTACCGCTCAATCTGACAACCCAC
PUREAFSpeICGTAACTAGTAGTCGTGGCCACCATTATCA
PUREARBamHIGTAGGATCCATCAAGGTTGGATGTAATTG
AAHPCBFPstIATGCCTGCAGGCTGATTGAGTGGAAAGCATA
AAHPCB670RGATACTCTGCAACGCCTTGGT
  • a Restriction sites are underlined in the oligonucleotide sequences.