TABLE 1.

Strains and plasmids

Strain or plasmidDescriptionaSource or reference
S. aureus strains
    RN4220Mcs; restriction-negative derivative of 8325-4R. Novick
    COLHomogeneous Mcr Ems TcsThe Rockefeller University Collection
    COL-SCOL with SCCmec excised; McsThis study
    COL-SspacP1COL-S with pbpA gene under Pspac control in the chromosome; EmrThis study
    COLspacP1COL with pbpA gene under Pspac control in the chromosome; EmrThis study
    COLpSK5632COL with pSK5632; CmrThis study
    COLspacP1pSKP1COLspacP1 with pSKP1; Emr CmrThis study
    LH6078325-4; spa TcrS. Foster
E. coli strain
    DH5αrecA endA1 gyrA96 thi-1 hsdR17 supE44 relA1 φ80ΔlacZΔM15Invitrogen
Plasmids
    pSRTemperature-sensitive vector pYT3 with ccrA and ccrB; Tcr14
    pMGPIS. aureus integrative vector including the IPTG-inducible Pspac promoter and lacI gene; Apr Emr25
    pSK5632E. coli-S. aureus shuttle vector containing the lac promoter; Apr Cmr8
    pMGPApMGPI vector with pbpA ribosome-binding site and the first 277 codons fused to the Pspac promoter; Apr EmrThis study
    pSKP1pSK5632 vector with pbpA complete coding sequence; Apr CmrThis study
  • a Abbreviations: Mcs, methicillin susceptible; Mcr, methicillin resistant; Ems, erythromycin susceptible; Tcs, tetracycline susceptible; Emr, erythromycin resistant; Cmr, chloramphenicol resistant; Apr, ampicillin resistant.