TABLE 1.

Dry weights of biofilm and planktonic fractions

Isolated fractionDry wt, g sample/g cells (actual value [g])aB/P ratiob
BiofilmPlanktonic
Biofilm1.1458 (1.1458 ± 0.0593)NAc NA
Cells1 (0.9064 ± 0.0321)1 (0.8287 ± 0.0468)1
Matrix0.1267 (0.1148 ± 0.0129)NANA
Ultracentrifuged pellet0.0231 (0.0209 ± 0.0013)0.0071 (0.0059 ± 0.0011)3.25
Washed pellet0.0064 (0.0058 ± 0.0025)0.0013 (0.0011 ± 0.0003)4.92
Wash supernatant0.0213 (0.0193 ± 0.0013)0.0050 (0.0041 ± 0.0002)4.26
  • a Dry weights are the averages of six readings ± standard errors of the means and were obtained from populations of similar CFU.

  • b B/P ratio refers to the biofilm/planktonic dry weight ratio of the values that have been normalized to their respective g cell weight. The B/P ratio for the two Kdo-rich (i.e., MV-rich; washed pellet for planktonic cells and wash supernatant for biofilm) fractions was 16.38.

  • c NA, not applicable.