TABLE 2.

Transcriptional activation of bfeA by catecholamines

Compoundβ-Galactosidase activity (Miller units) of B013N(pMP3) (bfeA-lacZ):Fold inductiona
Without FeWithout Fe and with compound (50 μM)
Norepinephrine32.1 (±27.8)b904.7 (±61.0)28.2
Carbidopa55.2 (±3.8)1,388.1 (±257.4)25.1
Isoproterenol55.2 (±3.8)1,375.5 (±209.7)24.6
Phenylephrine38.4 (±9.2)24.1 (±15.2)c
Tyramine38.4 (±9.2)43.1 (±17.0)1.1
  • a LacZ activity without Fe and with compound ÷ LacZ activity without Fe.

  • b ±1 standard deviation.

  • c −, no induction.