TABLE 2.

List of plasmids and strains used in this study

Plasmid or strainFeatures and applicationReference or source
Plasmids
    pTAD57Promoterless 980 bp xylE gene cloned into the SmaI site of pKS (in Escherichia coli DH5α)31
    pSAP112msr (hp0224) cloned into the NdeI-XhoI site of pET21A for overexpressionThis study
    pSAP120200-bp intergenic region of hp0223 and hp0224 (msr) cloned into the SacI and BamHI site of pTAD57 upstream of xylEThis study
    pSAP123The 1,180-bp PmsX (Pmsr-xylE) fragment cloned into the EcoRV site of peu39cm used in induction studiesThis study
    pSAP131The 1,180-bp PmsX fragment cloned into the BamHI site of pHel3 used in induction studiesThis study
    pSAP134The 1,200-bp PureA and xylE gene fusion fragment cloned into the BamHI site of pHel3This study
    pSAP138Thioredoxin (hp0824) cloned into NdeI-XhoI site of pET21A for overexpressionThis study
    pSAP139Thioredoxin reductase (hp0825) cloned into NdeI-XhoI site of pET21A for overexpressionThis study
    pSAP159Thioredoxin-2 (hp1458) cloned into the NdeI-XhoI site of pET21A for overexpressionThis study
    pSAP163Site-specific recombinase (hp1009) cloned into NdeI-XhoI site of pET21A for overexpression of a C-terminus six-His-tagged SSRThis study
    pILL690nikR (hp1338) inactivated with aphA311
Strains
    ALM 1014H. pylori SS1 carrying pSAP 123
    ALM 1018H. pylori SS1 carrying pSAP131 for induction studies
    PBR 0126H. pylori SS1 with pHel3 carrying promoterless xylE6
    ALM 1020pSAP123 recombined in hp0405 locus of msr::aphA3 in SS1This study
    ALM 1021H. pylori SS1 carrying pSAP134 as a positive control for induction studiesThis study
    ALM 1032pSAP123 recombined in the hp0405 locus of fur::aphA3 in SS1This study
    ALM 1035pILL690 transformed into SS1 to generate nikR::aphA3This study
    ALM 1037pSAP123 recombined in hp0405 locus of ALM1035This study