TABLE 4.

Cofactor preferences of different dehydrogenases in PKP and in final-product formation of glucose- or sucrose- grown L. reuteri ATCC 55730a

EnzymeActivity (μmol·min−1 mg protein−1) on:
Glucose Sucrose
NADPHNADHNADPHNADH
G6PDH2.3 ± 0.090.73 ± 0.040.87 ± 0.020
6PGDH0.64 ± 0.000.33 ± 0.010
LDH016 ± 0.309.3 ± 0.2
ADH2.4 ± 0.111 ± 21.0 ± 0.13.9 ± 0.6
  • a Specific activities of G6PDH, 6PGDH, LDH, and alcohol dehydrogenase (ADH). Values are the means ± standard deviations of the results of triplicate experiments.