TABLE 1.

Bacterial strains and plasmids used in this study

Bacterial strain or plasmidGenotype or markers and characteristics and usesSource or reference
Strains
    Shewanella sp. strain ANA-3As(V)-respiring gram-negative bacterium isolated from a wooden pier in Eel Pond, Woods Hole, MA16
    E. coli
        UQ950DH5α λ(pir) lysogen of DH5α used for cloningD. Lies, Caltech
        C43Mutant of overexpression strain BL21 optimized for expression of membrane and toxic proteins10
Plasmids
    pET32HModified version of pET32 (Novagen) in which sequences encoding the S tag and enterokinase cleavage site were removed22
    pET32H Xa-2Modified version of pET32H in which the sequence encoding the thrombin cleavage site was replaced with a factor Xa cleavage site and the His6 tag was replaced with a His9 tagThis study
    pET32HPModified version of pET32H in which the sequence encoding the thrombin cleavage site was replaced with a Prescission protease cleavage siteL. Dietrich, Caltech