TABLE 4.

Growth of sensor kinase mutants under various stress conditions

StrainGrowth under various stress conditionsa
Oxidative stressSuperoxide stressDisulfide stressAcid stressThermal stressOsmotic stressDNA damageAntibiotic stressMutacin production
UA159 (wild type)+++++++++
IBS340 (Δsmu45)++++++-++
IBS341 (Δsmu486)-*b+++-*+++-*c
IBS342 (Δsmu577)++++++++d+
IBS343 (Δsmu660)++++++++d+
IBS344 (Δsmu928)+++++++++
IBS345 (Δsmu1009)+++++++-e+
IBS346 (Δsmu1037)+++++++++
IBS347 (Δsmu1128)-*b-f+-*-*+-*+-*c
IBS348 (Δsmu1145)+++++++++
IBS349 (Δsmu1516)+++++g++-*h+
IBS350 (Δsmu1548)+++++++++
IBS351 (Δsmu1814)+++++++++
IBS352 (Δsmu1516)+++++++-e-i
IBS353 (Δsmu1965)+++++++++
  • a Multiple conditions/reagents were used to generate these stresses. *, Wild-type phenotypes were restored upon complementation of the mutant strain with the wild-type gene in trans.

  • b Refer to Table 3.

  • c Mutacin production was reduced more than 100-fold with respect to the wild type.

  • d Higher resistance to mezlocillin was observed compared to the wild type.

  • e Sensitive to bacitracin only.

  • f Sensitive to paraquat but not to cumene hydroperoxide or t-BOOH.

  • g ∼10-fold-higher resistance was observed compared to the wild type.

  • h Sensitive to cefotaxime, cefuroxime, ceftazidime, meropenem, mezlocillin, penicillin, and trimethoprim.

  • i No mutacin production was observed.