TABLE 1.

Bacterial strains and plasmids

Strain or plasmidGenotype or constructionSource or reference
E. coli strains
    DH5αsupE44 ΔlacU169 (φ80 lacZΔM15) recA1 endA1 hsdR17 thi-1 gyrA96 relA1Invitrogen
    CC118 λpirΔ(ara-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE(Am) recA1 λpir phage lysogen12
P. putida strains
    PaW85Wild-type, carries Tn4652 in chromosome, isogenic to completely sequenced KT24403
    PaW85tetPaW85 derivate, Tn4652 tnpA and tnpC genes are knocked out with Tcr gene (Tcr)30
    PaW4652KmPaW85 derivate, Tn4652 carries Kmr gene between positions 10920 and 11477 from the right end of transposon (Kmr)This study
Plasmidsa
    pAYC32Broad-host-range cloning vector (Apr)6
    pEST1332pAYC32 containing promoterless pheBA genes (Apr)18
    pEST2331pAYC32 containing promoterless pheBA genes as a 4.3-kb Ecl136II-PvuII fragment between Ecl136II and BamHI restriction sites (Apr)This study
    pPR9TTBroad-host-range vector containing lacZ without ATG (Cmr Apr)28
    pBluescript KSE. coli cloning vector (Apr)Stratagene
    pGP704LDelivery plasmid for homologous recombination (Apr)24
    pUTmini-Tn5Km2Delivery plasmid for mini-Tn5Km2 (Apr Kmr)9
    pKS/4652pBluescript KS containing 1.6-kb HindIII/ClaI Tn4652 fragment (Apr)This study
    pKS/4652KmpKS/4652 with Kmr in Tn4652 fragment (Apr Kmr)This study
    pGP704L/4652KmpGP704L containing 1.6-kb HindIII/ClaI Tn4652 fragment with Kmr (Apr Kmr)This study
    pRK2013Helper plasmid for conjugal transfer of pGP704L (Kmr)10
  • a Oligonucleotides used for the construction of plasmids are presented in Table S1 in the supplemental material.