TABLE 1.

Primers used in this study

PrimerSequence
PlinkRB5′-AAGGTCCATGCCTTCCTCC-3′/5′-TCTTTGTCACTATCTCGAAACCAA-3′
PlinkBC5′-AGGTCCATGCCTTCCTCC-3′/5′-CCCGTGCTCATAGCCCTA-3′
PlinkCC5′-CCGCCAAAGGGTTTAGTC-3′/5′-CGGGAGACAAGAAGAAACTC-3′
ParsGSP15′-GAAGCTGGATGACCTTCTCG-3′
ParsGSP25′-GAACGGGATGCCAAGATAGA-3′
ParsGSP35′-CTTGACGTCCGGGTACAGAT-3′
PdTanchor5′-GACCACGCGTATCGATGTCGACTTTTTTTTTTTTV-3′
Panchor5′-GACCACGCGTATCGATGTCGAC-3′
ParsC15′-GGTTCATGTTCGCCTCTGCC-3′/5′-GGCCGTCTCTTTCGCGCTG-3′
ParsRBCC5′-CCATTAGCTTGTTGCTACACC-3′/5′-GCCAAGGTGCTAATGAAATGA-3′
ParsC1RTa5′-GGTTCATGTTCGCCTCTGCC-3′
ParsRRTa5′-CGCAGCACGTTCAAAACTC-3′
ParsBRTa5′-GACATGACAGACACCGGCAA-3′
ParsC2RTa5′-CACCGAATTTGTCACAACGC-3′
ParsC3RTa5′-TCGATGGTCTTTGACTCTTGC-3′
P16SRTa5′-CTGCTGGCACGGAGTTAGC-3′
ParsC1RealTb5′-GTCAAAGCTGGTTTCCGAACTG-3′/5′-GGCTTTCGGCAGGAAAAAA-3′
ParsRRealTb5′-TCACAGAAGCCTTAGCTTTGC-3′/5′-ATCCATTTTCCGCTCCTTCT-3′
ParsBRealTb5′-ATCGCCATCGGCTTGATTC-3′/5′-GACAATGCGAGCACCTTGAA-3′
ParsC2RealTb5′-ATTCCGCTGGTGTGAAAAAG-3′/5′-TCGATGGTCTTTGACTCTTGC-3′
ParsC3RealTb5′-ACTCCTGTCGAAGCCAGATG-3′/5′-GGGTTTAGTCCGTGCTTTTTC-3′
P16SRealTb5′-GGGTGAGTAACGCGTGGATT-3′/5′-AGCAGAGGCCCCCTTTACC-3′
  • a Primers were generated manually based on the sequence at the end of each gene.

  • b Primers were generated by Primer Express (ABI Prism).