TABLE 1.

B. subtilis strains

StrainGenotypeSource or reference
JH642trpC2 pheA1J. Hoch
MAB160trpC2 pheA1 ΩcitB::spc6
EUDC9901trpC2 pheA1 gerE::kanC. Moran (7)
JHBS5trpC2 pheA ΔspoIVCBJ. Haraldsen
JCB36trpC2 pheA1 ΩcitB::spc ΦspoIIA-lacZ cat6
JCB63trpC2 pheA1 ΩcitB::spc SPβc2 Δ2::pSK10Δ6::Φ(cotA-lacZ) tet6
SJB227trpC2 pheA1 SPβc2 Δ2::pSK10Δ6::Φ(sspB-lacZ) catS. Jin
BB1783trpC2 pheA1 citB-His6 neoB. Belitsky
AWS96trpC2 pheA1MAB160 × DNA JH642
AWS133trpC2 pheA1 citB5 (R740E Q744E F661L I809T V852A)AWS96 × pAWS42
AWS137trpC2 pheA1 citB5 (R740E Q744E F661L I809T V852A) SPβc2 Δ2::pSK10Δ6::Φ(cotA-lacZ) tetAWS133 × DNA JCB63
AWS144trpC2 pheA1 citB-His6 neoAWS96 × DNA BB1783
AWS145trpC2 pheA1 citB-His6 neo SPβc2 Δ2::pSK10Δ6::Φ(cotA-lacZ) tetAWS144 × DNA JCB63
AWS149trpC2 pheA1 ΩcitB::spc ΦspoIIA-lacZ tetJCB36 × pP528
AWS153trpC2 pheA1 citB5 (R740E Q744E F661L I809T V852A) SPβc2 Δ2::pSK10Δ6::Φ(spoIIAA-lacZ) tetAWS133 × DNA AWS149
AWS154trpC2 pheA1 citB-His6 neo SPβc2 Δ2::pSK10Δ6::Φ(spoIIAA-lacZ) tetAWS144 × DNA AWS149
AWS198trpC2 pheA1 His10-citB catJH642 × pAWS58