TABLE 1.

B. subtilis strains

StrainGenotypeSource or reference
JH642 trpC2 pheA1 J. Hoch
MAB160 trpC2 pheA1 ΩcitB::spc 6
EUDC9901 trpC2 pheA1 gerE::kan C. Moran (7)
JHBS5 trpC2 pheA ΔspoIVCB J. Haraldsen
JCB36 trpC2 pheA1 ΩcitB::spc ΦspoIIA-lacZ cat 6
JCB63 trpC2 pheA1 ΩcitB::spc SPβc2 Δ2::pSK10Δ6::Φ(cotA-lacZ) tet 6
SJB227 trpC2 pheA1 SPβc2 Δ2::pSK10Δ6::Φ(sspB-lacZ) cat S. Jin
BB1783 trpC2 pheA1 citB-His6 neo B. Belitsky
AWS96 trpC2 pheA1 MAB160 × DNA JH642
AWS133 trpC2 pheA1 citB5 (R740E Q744E F661L I809T V852A)AWS96 × pAWS42
AWS137 trpC2 pheA1 citB5 (R740E Q744E F661L I809T V852A) SPβc2 Δ2::pSK10Δ6::Φ(cotA-lacZ) tet AWS133 × DNA JCB63
AWS144 trpC2 pheA1 citB-His6 neo AWS96 × DNA BB1783
AWS145 trpC2 pheA1 citB-His6 neo SPβc2 Δ2::pSK10Δ6::Φ(cotA-lacZ) tet AWS144 × DNA JCB63
AWS149 trpC2 pheA1 ΩcitB::spc ΦspoIIA-lacZ tet JCB36 × pP528
AWS153 trpC2 pheA1 citB5 (R740E Q744E F661L I809T V852A) SPβc2 Δ2::pSK10Δ6::Φ(spoIIAA-lacZ) tet AWS133 × DNA AWS149
AWS154 trpC2 pheA1 citB-His6 neo SPβc2 Δ2::pSK10Δ6::Φ(spoIIAA-lacZ) tet AWS144 × DNA AWS149
AWS198 trpC2 pheA1 His10-citB cat JH642 × pAWS58