TABLE 1.

Bacterial strains and plasmids used in this study

Strain(s) or plasmidDescriptionReference or source
K. pneumoniae strains
    Clinical isolates74 isolates collected from National Taiwan University Hospital from 1997 to 200318
    NTUH-K2044Clinical strain causing PLA and meningitis18
    MGH78578ATCC 700721, used for determination of the complete genome sequence at Washington UniversityATCC
    NTUH-K2044 int mutantNTUH-K2044 isogenic int mutant (insertion mutation)This study
    NTUH-K2044 irp2 mutantNTUH-K2044 isogenic irp2 mutant (irp2 replaced by a kanamycin resistance gene)This study
    NTUH-K2044 rmpA mutantNTUH-K2044 isogenic rmpA mutant (mutant 3-20, transposon insertion mutant)18
    NTUH-K2044 virB1 mutantNTUH-K2044 isogenic virB1 mutant (insertion mutation)This study
    NTUH-K2044 mobB mutantNTUH-K2044 isogenic mobB mutant (insertion mutation)This study
    N4252 RifrNon-tissue-invasive strain, spontaneous rifampin-resistant mutantThis study
E. coli strains
    HB101supE44 hsdS20(rB mB) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 leuB6 thi-1Takara
    CC118λpirΔ(ara-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE(Am) recA1 λpir phage lysogen23
    S17-1λpirhsdR recA pro RP4-2 (Tc::Mu; Km::Tn7)(λpir)31
Plasmids
    pACYC184Cloning vector, P15A replicon, CmrNew England Biolabs
    pUT-kmpUTKm1-derived plasmid with mini-Tn5 excised by EcoRI, tnp excised by SalI, and bla removed by ApaLI and with insertion of a kanamycin resistance cassette from pUC4K into a PstI site17