Bacterial strains and plasmids used in this study

Strain(s) or plasmidDescriptionReference or source
K. pneumoniae strains
    Clinical isolates74 isolates collected from National Taiwan University Hospital from 1997 to 200318
    NTUH-K2044Clinical strain causing PLA and meningitis18
    MGH78578ATCC 700721, used for determination of the complete genome sequence at Washington UniversityATCC
    NTUH-K2044 int mutantNTUH-K2044 isogenic int mutant (insertion mutation)This study
    NTUH-K2044 irp2 mutantNTUH-K2044 isogenic irp2 mutant (irp2 replaced by a kanamycin resistance gene)This study
    NTUH-K2044 rmpA mutantNTUH-K2044 isogenic rmpA mutant (mutant 3-20, transposon insertion mutant)18
    NTUH-K2044 virB1 mutantNTUH-K2044 isogenic virB1 mutant (insertion mutation)This study
    NTUH-K2044 mobB mutantNTUH-K2044 isogenic mobB mutant (insertion mutation)This study
    N4252 RifrNon-tissue-invasive strain, spontaneous rifampin-resistant mutantThis study
E. coli strains
    HB101supE44 hsdS20(rB mB) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 leuB6 thi-1Takara
    CC118λpirΔ(ara-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE(Am) recA1 λpir phage lysogen23
    S17-1λpirhsdR recA pro RP4-2 (Tc::Mu; Km::Tn7)(λpir)31
    pACYC184Cloning vector, P15A replicon, CmrNew England Biolabs
    pUT-kmpUTKm1-derived plasmid with mini-Tn5 excised by EcoRI, tnp excised by SalI, and bla removed by ApaLI and with insertion of a kanamycin resistance cassette from pUC4K into a PstI site17