TABLE 1.

E. coli strains and plasmids used in this study

Strain plasmidDescription and featuresReference or source
Vibrio cholerae strains
    O395O1 classical, VPI-2+, VSP-I and VSP-II deficientLaboratory collection
    2740-80O1 El Tor, VPI-2+, VSP-I and VSP-II deficientLaboratory collection
    N16961O1 El Tor, VPI-2+, VSP-I+, VSP-II+Laboratory collection
    E4O1 El Tor, E7946 derivativeLaboratory collection
    SG6Non-O1, VPI-2 deficient, VSP-I deficient, VSP-II deficientLaboratory collection
    MO2O139, VPI-2+, VSP-I+, VSP-II+Laboratory collection
    MO10O139, VPI-2+, VSP-I+, VSP-II+Laboratory collection
    Bah-3E7946 ΔrecALaboratory collection
    RAM-1N16961 ΔintVPI 2This study
    RAM-2N16961 ΔintVSP IThis study
    RAM-3N16961 ΔintVSP IIThis study
Escherichia coli strains
    DH5αλpir
    β2155ΔDAP pir
    RM1DH5αλpir(pRM1)This study
    RM2DH5αλpir(pRM2)This study
    RM3DH5αλpir(pRM3)This study
    RM4β2155(pRM1)This study
    RM5β2155(pRM2)This study
    RM6β2155(pRM3)This study
    RM7DH5αλpi(pRM4)This study
    RM8DH5αλpi(pRM5)This study
    RM10β2155(pRM4)This study
    RM11β2155(pRM5)This study
Plasmids
    pDS132cat sacB Δpir27
    pRM1pDS132 intVPI 2 SOE constructThis study
    pRM2pDS132 intVSP I SOE constructThis study
    pRM3pDS132 intVSP II SOE constructThis study