TABLE 5.

Quantification of the induction by heat shock of selected genes in wild-type and rpoE2 mutant cells of S. meliloti

GeneInduction (n-fold) after heat shock (±SD)a in strain:
Rm1021 (wild type)CBT208 (rpoE2)
RpoE2-dependent
    sma01136.2 (±1.1)1.5 (±0.5)
    sma013421.4 (±14.5)1.2 (±0.4)
    smb20007 (katC)29.5 (±13.9)1.5 (±0.6)
    smb2009436.7 (±23.7)1.3 (±0.4)
    smb20227 (ndiA1)16.7 (±14.2)1.1 (±0.4)
    smb21484 (rpoE5)59.7 (±17.7)0.5 (±0.2)
    smc00371130.5 (±100.1)1.4 (±0.7)
    smc00885153.6 (±93.5)1.2 (±0.3)
    smc01504106.7 (±72.1)1.6 (±0.6)
    smc0150530.7 (±7.0)1.3 (±0.5)
    smc03873 (rpoH2)21.2 (±9.7)1.6 (±0.4)
Independent or partially dependent on RpoE2
    sma0612 (fixN3)4.8 (±2.5)4.3 (±1.4)
    smb214568.4 (±5.4)4.6 (±1.3)
    smb21566 (groEL5)b124.7 (±123.5)223.7 (±88.8)
    smc011403.3 (±1.5)3.2 (±1.0)
    smc0126710.9 (±4.3)7.8 (±3.1)
    smc018145.3 (±3.9)8.3 (±2.6)
    smc028854.2 (±2.2)3.6 (±2.7)
  • a Measured by qRT-PCR as described in Materials and Methods. The results are the averages of at least three independent biological experiments.

  • b Although designed to amplify groEL5, the primers used for PCR may also amplify other groEL genes that are highly homologous to groEL5.