TABLE 1.

Bacterial strains and plasmidsa

Strain or plasmidRelevant characteristicsReference or source
E. coli strains
    BL21F ompT hsdSΒ(rΒ mΒ) gal dcm tonANovagen
    Top10Cloning strain, F mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupGInvitrogen
    SK0140Plasmid mobilization strain; Top10 with plasmid RK21761 (RK2lac oriT); Apr Kmr Tcs Lac+69
    MT1064BL21 with pMT137 (flp-1); CmrThis work
    MT1078BL21 with pMT137 (flp-1) and pSK305 (tadV); Cmr TcrThis work
A. actinomycetemcomitans strains
    CU1000NSpontaneous nalidixic acid-resistant mutant of rough clinical isolate CU1000 (25), serotype f; Nalr40
    DF2200NSpontaneous nalidixic acid-resistant mutant of rough clinical isolate DF2200, serotype a; Nalr44
    Aa0886CU1000N tadZΩ::IS903φkan; Kmr Nalr62
    Aa1332CU1000N tadBΩ::IS903φkan; Kmr Nalr40
    Aa1347CU1000N tadEΩ::IS903φkan; Kmr Nalr40
    Aa1354CU1000N rcpAΩ::IS903φkan; Kmr Nalr62
    Aa1359CU1000N tadCΩ::IS903φkan; Kmr Nalr40
    Aa1360CU1000N tadAΩ::IS903φkan; Kmr Nalr40
    Aa1361CU1000N rcpCΩ::IS903φkan; Kmr Nalr62
    Aa1512CU1000N tadFΩ::IS903φkan; Kmr Nalr40
    Aa1561CU1000N tadGΩ::IS903φkan; Kmr Nalr40
    Aa1577CU1000N tadDΩ::IS903φkan; Kmr Nalr40
    Aa2508Aa3074 with pGS-GFP-4; Cmr Kmr NalrThis work
    Aa2512Aa1512 with pGS-GFP-4; Cmr Kmr NalrThis work
    Aa2513Aa1512 with pMT115; Cmr Kmr NalrThis work
    Aa2515Aa1512 with pMT127 (tadF E23A); Cmr Kmr NalrThis work
    Aa2519CU1000N with pJAK17; Cmr Kmr NalrThis work
    Aa2530JK1010 with pMT143 (flp-1 E31A); Cmr Kmr NalrThis work
    Aa2531Aa1512 with pJAK17; Cmr Kmr NalrThis work
    Aa2532Aa1512 with pMT141 (wild-type tadF); Cmr Kmr NalrThis work
    Aa2534Aa1347 with pJAK17; Cmr Kmr NalrThis work
    Aa2535JK1010 with pMT137 (wild-type flp-1); Cmr Kmr NalrThis work
    Aa2536JK1010 with pMT138 (flp-1 G26A); Cmr Kmr NalrThis work
    Aa2540Aa3074 with pJAK17; Cmr Kmr NalrThis work
    Aa2549Aa1512 with pMT150 (tadF G18A); Cmr Kmr NalrThis work
    Aa2550Aa1347 with pMT148 (tadE G10A); Cmr Kmr NalrThis work
    Aa2552JK1010 with pMT156 (flp-1 G26Y); Cmr Kmr NalrThis work
    Aa2553Aa1347 with pMT157 (wild-type tadE); Cmr Kmr NalrThis work
    Aa2559Aa2559 with pMT158 (tadE E15A); Cmr Kmr NalrThis work
    Aa2561Aa3074 with pMT163 (tadV D77A); Cmr Kmr NalrThis work
    Aa2565Aa3074 with pMT168 (tadV D23A); Cmr Kmr NalrThis work
    Aa2563Aa1512 with pMT164 (tadF G18Y); Cmr Kmr NalrThis work
    Aa2567Aa1347 with pMT169 (tadE G10Y); Cmr Kmr NalrThis work
    Aa2569Aa3047 with pMT170 (wild-type tadV); Cmr Kmr NalrThis work
    Aa2575Aa1347 with pMT176 (flp-1-tadE chimera); Cmr Kmr NalrThis work
    Aa2579Aa1512 with pMT177 (flp-1-tadF chimera); Cmr Kmr NalrThis work
    Aa3074DF2200N tadV::mini-Tn5Km; Kmr Nalr59
    JK1010CU1000N flp-1Ω::IS903φkan; Kmr Nalr42
Plasmids
    pCF430Broad-host-range IncP expression vector with arabinose-inducible promoter; araC araBADp lacIq; Tcr53
    pCR2.1-TOPOTA cloning vector; Apr KmrInvitrogen
    pCR4-TOPOTA cloning vector; Apr KmrInvitrogen
    pGS-GFP-04gfp cloned in pMMB20731
    pJAK17Broad-host-range IncQ expression vector; derivative of pMMB67HE with Ωcat insertion in bla; lacIq tacp mob+; CmrJ. A. Kornacki, unpublished
    pMT115tadF-egfp fusion in pJAK17; CmrThis work
    pMT127tadF E23A in pJAK17; CmrThis work
    pMT137flp-1 in pJAK17; CmrThis work
    pMT138flp-1 G26A in pJAK17; CmrThis work
    pMT141tadF in pJAK17; CmrThis work
    pMT143flp-1 E31A in pJAK17; CmrThis work
    pMT148tadE G10A in pJAK17; CmrThis work
    pMT150tadF G18A in pJAK17; CmrThis work
    pMT156flp-1 G26Y in pJAK17; CmrThis work
    pMT157tadE in pJAK17; CmrThis work
    pMT158tadE E15A in pJAK17; CmrThis work
    pMT163tadV D77A in pJAK17; CmrThis work
    pMT164tadF G18Y in pJAK17; CmrThis work
    pMT168tadV D23A in pJAK17; CmrThis work
    pMT169tadE G10Y in pJAK17; CmrThis work
    pMT170tadV in pJAK17; CmrThis work
    pMT176flp-1-tadE chimera in pJAK17; CmrThis work
    pMT177flp-1-tadF chimera in pJAK17; CmrThis work
    pSK305tadV in pCF430; TcrThis work
  • a Abbreviations: Ap, ampicillin; Cm, chloramphenicol; Km, kanamycin; Nal, nalidixic acid; Tc, tetracycline.