TABLE 3.

MccE492 sensitivities of mannose permease-deficient strains and other strainsa

StrainRelevant genotypeActivity ± SD (AU/ml)b
DB503Wild type960 ± 111
MG1655Wild type939 ± 148
JCB606Wild type896 ± 222
AB4 manY::Tn10 No activity
AB5 manY::Tn10 No activity
AB6 manY::Tn10 No activity
AB3ΔmanXYZ No activity
AB3(pJFL)ΔmanXYZ/manX + No activity
AB3(pTSP11)ΔmanXYZ/manY + No activity
AB3(pAB17)ΔmanXYZ/manZ + No activity
AB3(pJFP-H6M)ΔmanXYZ/manYZ + 1045 ± 225
AB3(pJFLPM)ΔmanXYZ/manXYZ + 896 ± 222
MG1655(pAB8)Wild type939 ± 148
MG1655(pAB7) mceB + No activity
H1443Wild type960 ± 111
H1876 fepA fiu cir No activity
AB2Wild type1045 ± 225
AB1 tonB 48 ± 16
  • a The bactericidal activity of purified MccE492 (10 mg/ml) was assessed in triplicate for the indicated strains by the critical dilution method (see Materials and Methods). Expression from the indicated plasmids was induced with 600 μM IPTG or 0.2% arabinose for Ptac or PBAD promoters, respectively.

  • b AU, arbitrary units.