Effect of dppA promoter mutations on AbrB-dependent regulation of dppA-lacZ fusionsa
Strain | Relevant genotypeb | Fusion genotype | β-Galactosidase activity | |
---|---|---|---|---|
MU | %c | |||
BB2681 | codY | dppAp+ | 155.0 | 41 |
BB2786 | codY abrB | 374.0 | 100 | |
BB2744 | codY | dppAp50 | 281.0 | 90 |
BB2787 | codY abrB | 312.0 | 100 | |
BB2684 | codY | dppAp51 | 264.0 | 78 |
BB2788 | codY abrB | 338.0 | 100 | |
BB2707 | codY | dppAp54 | 124.0 | 33 |
BB2789 | codY abrB | 375.0 | 100 | |
BB2708 | codY | dppAp55 | 151.0 | 46 |
BB2790 | codY abrB | 330.0 | 100 |
↵a All strains were grown in TSS glucose-ammonium medium with a mixture of 16 amino acids. β-Galactosidase activity (Miller units [MU]) was assayed as described for Table 2.
↵b All strains contained a lacA null mutation that eliminated endogenous β-galactosidase activity (9).
↵c The β-galactosidase activity of each fusion in a strain containing codY and abrB null mutations was normalized to 100%.