TABLE 6.

Effect of dppA promoter mutations on AbrB-dependent regulation of dppA-lacZ fusionsa

StrainRelevant genotypebFusion genotypeβ-Galactosidase activity
MU%c
BB2681codYdppAp+155.041
BB2786codY abrB374.0100
BB2744codYdppAp50281.090
BB2787codY abrB312.0100
BB2684codYdppAp51264.078
BB2788codY abrB338.0100
BB2707codYdppAp54124.033
BB2789codY abrB375.0100
BB2708codYdppAp55151.046
BB2790codY abrB330.0100
  • a All strains were grown in TSS glucose-ammonium medium with a mixture of 16 amino acids. β-Galactosidase activity (Miller units [MU]) was assayed as described for Table 2.

  • b All strains contained a lacA null mutation that eliminated endogenous β-galactosidase activity (9).

  • c The β-galactosidase activity of each fusion in a strain containing codY and abrB null mutations was normalized to 100%.