TABLE 5.

Expression of dppA-lacZ fusionsa

StrainRelevant genotypebFusion genotypeAddition to medium (no. of aa)β-Galactosidase activity
MU%c
BB2781Wild typedppAp+None115.031
1341.711
166.31.7
BB2786codYNone419.0112
16374.0100
BB2782Wild typedppAp50None344.0110
13310.099
16251.080
BB2787codY16312.0100
BB2783Wild typedppAp51None366.0108
13307.091
16182.054
BB2788codY16338.0100
BB2784Wild typedppAp54None309.082
13203.054
1669.018
BB2789codY16375.0100
BB2785Wild typedppAp55None1.20.4
130.90.3
160.10.04
BB2790codY16330.0100
  • a Cells were grown and β-galactosidase activity (Miller units [MU]) was assayed as described for Table 2.

  • b All strains contained an abrB null mutation (45) and a lacA null mutation that eliminated endogenous β-galactosidase activity (9).

  • c The β-galactosidase activity of each fusion in the 16-aa-containing medium in a strain containing a codY null mutation was normalized to 100%.