TABLE 6.

ORFs deduced from the genome of S. thermophilus phage 858 and their predicted functions

ORFStartStopSize (aa)aMass (kDa)pIPutative ribosome-binding site and start codon (AAAGGAGGTGA)Best matchesb (% amino acid identity)Sizec (aa)Putative function
1319525688.110.5Not identified ATGgp68 phage Sfi11 67/68 (98)68
241482713716.28.7CAGAGAGGTTAgtaca ATGORF1 phage 2972 137/137 (100)137
31009146115016.86.7AAAATATTTGCggatgaatac TTGORF2 phage 2972 145/150 (96)150Terminase small subunit
41448211922325.39.0AAAGGAGCTGTaagcg ATGORF3 phage 2972 223/223 (100)223Terminase large subunit
52412299919522.74.8AGAGCCCTTGCgataacaaata ATGORF4 phage 2972 194/195 (99)195Terminase large subunit
63008451350157.65.0GTAGGAGGAATg ATGORF5 phage 2972 501/501 (100)501Portal protein
74510540329734.48.8TTGAGAGGGAAtatga ATGORF6 phage 2972 294/297 (98)297Capsid protein
85591617219321.34.8TAGATAGGAGAaata ATGORF7 phage 2972 193/193 (100)193Scaffold protein
96192655111912.88.8AAAGGAAATTTtaa ATGORF8 phage 2972 115/119 (96)119Capsid protein
106570761634837.54.9AGAGGAGGAACattaaaac ATGORF9 phage 2972 346/348 (99)348Capsid protein
1176287789536.09.3TTAAGAGGTACtgat ATGORF10 phage 2972 52/53 (98)53
127801814211313.04.6TAGTGAGGTATggc ATGORF11 phage 2972 113/113 (100)113
138139845310411.59.5AAAGAGGGAGAggtgttatttct ATGORF12 phage 2972 104/104 (100)104
148455879311212.58.9CAAGGTGGTGAaataac ATGORF13 phage 2972 112/112 (100)112
158795918112814.65.0AATGGCTAAGTgggaataag ATGORF14 phage 2972 127/128 (99)128
169195970416918.54.8TCAGGAGGAAAaa ATGORF15 phage 2972 168/169 (99)168Tail protein
1797821013511713.24.7ATAGGAGTTAAaaaca ATGgp117 phage Sfi11 101/117 (86)117
18101861050310512.69.9CGAGGAATTAAtcactaatgct ATGORF17 phage 2972 104/105 (99)105
1910493150461517153.49.6AGAGGGGCTTGctag ATGORF18 phage 2972 1511/1517(99)1517Tail protein
20150461658151157.75.3ATGAGAGGTATtaaata ATGORF19 phage 2972 508/511 (99)511
2116581214341617178.55.3ATTTGAGGAGAgatatatata ATGORF20 phage 2972 1599/1617(98)1605Receptor-binding protein
22214352345667374.25.7GTAGGAGGTTTttaa TTGORF21 phage 2972 659/673 (97)673Structural protein
23234732385912814.64.7AAGAAAGGAAAaatat ATGORF22 phage 2972 126/128 (98)128
242388524028475.56.6TTAGGAGGAAGaaca ATGORF23 phage 2972 47/47 (100)47
2523991242789511.08.7CAAGAGCTTGTaggcttgtctc ATGT. parva 22/87 (25)497
262427524409445.010.7AAGCGAGGTTGataa ATGS. pyogenes MGAS5005 19/35 (54)61
272443724691849.39.5GAAACTTGAGAgg ATGORF87 phage Sfi21 68/79 (86)87Holin
28246932529520021.84.5TAAGGAAGGAAaatagt ATGORF25 phage DT1 180/200 (90)200Endolysin
292534125463404.68.8AAACAAAGCGGtgtc ATGORF27 phage 2972 40/40 (100)40
30255022626325330.09.7AAAGATGGTGTcataag ATGPhage J1 248/253 (98)253Endonuclease
312632526552758.63.9AATCCCGGTTAca ATGPhage J1 70/75 (93)186Endolysin
322672026851435.38.9AAACGAGGTGAaaaca ATGORF30 phage 2972 43/43 (100)43
332695227161697.97.9AAAGGAGAACTta ATGORF31 phage 2972 68/69 (98)69Cro-like repressor
342717827300405.08.0AAAGGTATTTAaa ATGORF32 phage 2972 40/40 (100)40
35275572803015718.16.2TAGGGAGGGTAggaattaaat ATGORF33 phage 2972 157/157 (100)157
36280272872823326.26.6AAAGGAGAAACcttaacataag ATGORF34 phage 2972 233/233 (100)233
37286853009747054.28.6AAAGGGGTGTAaggtag ATGORF35 phage 2972 309/473 (65)445Helicase
38301043057715718.24.8AGATTTGGAGAtaaaaaaac ATGS. suis 89/1591 110/157 (70)156
39305823139727130.77.8Not identified ATGS. suis 89/1591 180/263 (68)268
40313663291051460.08.0AAAGGAGTTAGatactaaac ATGS. suis 89/1591 363/508 (71)517Primase
41331673348710612.19.9AAAGAAAGGCAactttcaa GTGORF39 phage 2972 102/106 (96)106
423369833871576.68.1GAAAGAGATGAtagaact ATGORF17 phage O1205 44/57 (77)57
433386834023516.35.6GTAGGAGATTAgtagagtt ATGORF41 phage 2972 51/51 (100)51
44340243453617019.66.3TGAGGTGGAATag ATGORF42 phage 2972 170/170 (100)170DNA-binding protein
45345053483110812.19.2ATAGGAAAGGAaagatggtaa ATGORF43 phage 2972 108/108 (100)108
46348353554223527.79.1TGAGGAGTTATtaagac ATGORF44 phage 2972 234/235 (99)235
  • a That is, the number of amino acids (aa) of the predicted protein.

  • b If the host species is not mentioned, the phage infects a S. thermophilus strain.

  • c That is, the size in amino acids (aa) of the best-matched protein.