TABLE 4.

Expression of yurP-lacZ fusionsa

StrainRelevant genotypebFusion genotypeAddition to medium (no. of aa)β-Galactosidase activity
MU%c
BB2675Wild typeyurPp+None143.07.4
1334.81.8
161.60.1
BB2687codYNone2,170.0112
161,930.0100
BB2698Wild typeyurPp1None15.30.9
133.20.2
160.40.02
BB2709codY161,720.0100
BB2716Wild typeyurPp2None442.024
13180.09.8
1630.51.7
BB2717codY161,840.0100
BB2700Wild typeyurPp3None426.022
13133.06.9
16102.05.3
BB2711codY161,930.0100
  • a Cells were grown and β-galactosidase activity (Miller units [MU]) was assayed as described for Table 2.

  • b All strains contained a lacA null mutation that eliminated endogenous β-galactosidase activity (9).

  • c The β-galactosidase activity of each fusion in the 16-aa-containing medium in a strain containing a codY null mutation was normalized to 100%.