TABLE 2.

List of experimental conditions under which the expression of QS genes was examined

Condition no.Description
1M9 (supplemented with 0.1% CAA)a
2LB medium
3BHI medium
4THY medium
51/4-diluted LB
61/4-diluted BHI medium
71/4-diluted THY medium
8TSB-DC medium
9TSB-DC + 50 μM FeCl3 medium
10M9 + 100 mM NaCl
11M9 + 200 mM NaCl
12M9 + AI-2 (5%)
131/4-diluted LB + 150 mM NaCl
141/4-diluted LB + 0.5% mucin
151/4-diluted LB + 0.25 μg/ml tobromycin
161/4-diluted LB + 0.5 μg/ml tobromycin
171/4-diluted LB + AI-2 10%
181/4-diluted LB + phosphate-buffered saline
19M9 + 2.5% sputum extract
20M9 + 5% sputum extract
21M9 + 2.5 μM C4-HSL
22M9 + 5 μM C4-HSL
23M9 + 200 μg/ml salmon sperm DNA
24M9 + 0.5% mucin
25M9 with 0.1% glucose instead of 0.5% (carbon source restricted)
26M9 + 0.25 μg/ml tobromycin
27M9 + 0.5 μg/ml tobromycin
28TSB-DC + EDDA; microaerophilic condition
29TSBDC + 50 μM FeCl3; microaerophilic condition
30M9; microaerophilic condition
31M9 + 00 mM NaCl; microaerophilic condition
32TSB-DC medium + EDDA
331/4-diluted LB, AI-2 (5%) + mucin (0.5%)
34M9 + (C12-HSL) dodecanoyl-l-homoserine lactone (5 μM)b
35M9 + (C12-HSL) dodecanoyl-l-homoserine lactone (10 μM)
36M9 + phosphate-buffered saline
37M9 + AI-2 (10%)
38M9 + mucin (0.25%)
39M9-BMc
401/4-diluted LB-BM
41LB-BM
421/4-diluted BHI-BM
43BHI-BM
441/4-diluted THY-BM
45THY-BM
46TSBDC-BM
  • a All M9 media containing CAA supplement.

  • b The C12-HSL is different from 3-oxo-C12-HSL produced by P. aeruginosa LasI.

  • c Breathable membranes were used instead of mineral oil for sealing the multiwell plate.