TABLE 1.

Strains used in this study

StrainDescriptionReference or source
E. coli S17-1 thi pro hsdR hsdM + recA; integrated plasmid RP4-Tc::Mu-Km::Tn7 30
E. coli S17-1:pZMR100Plasmid vector used to confer Kmr on S17-1; used as prey for Kmr Bdellovibrio strains 23
E. coli S17-1:pKJE102pSET151 suicide plasmid containing pilA::Kmr and BamHI and XbaI fragments in conjugation donor strain S17-1; used for gene interruption in Bdellovibrio 3; this study
E. coli S17-1:pSB3000, pZMR100YFP (Clontech) cloned into plasmid pDEST3 cotransformed with pZMR100; used as luminescent prey for Kmr Bdellovibrio strainsP. J Hill, University of Nottingham (unpublished)
B. bacteriovorus HD100Type strain; genome sequenced 22, 31
B. bacteriovorus HD100 pilA/pilA::KmMerodiploid HD100 with wild-type and kanamycin-interrupted pilA This study
B. bacteriovorus pilA HI /pilA::KmMerodiploid HI derivative of HD100 with wild-type and kanamycin-interrupted pilA This study
B. bacteriovorus pilA HI::KmPrey-independent HD100 derivative with kanamycin-interrupted pilA onlyThis study
B. bacteriovorus fliC3::KmPrey-independent derivative of 109J fliC3::KmThis study