TABLE 3.

Suppression of Mre and MrdB lethality by multiple copies of sdiA

P1 lysateaNo. of colonies for:
PB103/pGB2 (wt/vector)PB103/pCX16 (wt/sdiA)
lacIZYA<>aph326496
mreBCD<>aph1149
mreB<>aph1b133
mreC<>aph1123
mreD<>aph0110
mrdB<>aph077
  • a Equal aliquots of saturated cultures were mixed with equal aliquots of each transducing lysate, the mixtures were plated on LB-Kan-spectinomycin, and the numbers of transductant colonies that appeared after incubation at 37°C were counted.

  • b This transductant yielded strain FB2sup/pGB2.