TABLE 4.

Growth rates, FtsZ levels, and viabilities of spherical derivatives

Medium and temp (°C)Result for PB103Result for TB28
Td (min)a[FtsZ]bGrowthcTd (min)a[FtsZ]bGrowthc
MreBCDMrdABMreBCDMrdAB
LB
    37341.0− − −− − −271.1− − −− − −
    30601.0− − −− − −431.0− − −− − −
    25100ND+ − −+ + +75ND− − −+ + +
M9
    37151ND+ − −+ + +60ND− − −+ + +
    303020.9+ + ++ + +750.8− − −+ + +
    25>500ND+ + ++ + +301ND+ + ++ + +
  • a Td as measured by culture OD600.

  • b FtsZ levels in PB103 and TB28 as measured by quantitative Western analyses. Values were normalized to the level in cells grown in LB at 30°C. ND, not determined.

  • c No (− − −), poor (+ − −), or good (+ + +) growth on solid media of Mre and Mrd depletion derivatives. Also, see Fig. S1 and S3 in the supplemental material.