TABLE 1.

Bacterial strains and plasmids

Strain or plasmidRelevant characteristicsSource or reference
Strains
    Escherichia coli DH5αF endA1 hsdR17(rK mK+) supE44 thi-1 λ gyrA96 relA1 Δ(lacZYA argF)U169 (Φ80 lacZΔM15) recA1Roche
    Clostridium cellulolyticum ATCC 35319Wild type30
Plasmids
    pPSVpJIR418 derivative carrying the promoterless CAT gene (catP); Emr24
    pGEM-T EasyCloning vector; AprPromega
    pSOS952E. coli-Clostridium shuttle vector (ColE1 and pIM13 replicons); Apr Emr; carries the adc-ctfA-ctfB operon transcribed from a modified thl promoter (two lac operators)29
    pPSVthlpPSVΩ (SmaI Sos-dir/SosE 259-bp PCR fragment carrying thiolase promoter)This study
    pPSV1pPSVΩ (SphI/SacI 1,030-bp fragment of cipC promoter cloned in pGEM-T Easy)This study
    pPSV1ΔCREpPSVΩ (SphI/SacI 1,016-bp fragment of cipC ΔCRE promoter cloned in pGEM-T Easy)This study
    pPSV2pPSVΩ (SphI/SacI 155-bp fragment of cipC promoter cloned in pGEM-T Easy)This study
    pPSV3pPSVΩ (SphI/SacI 701-bp fragment of cipC promoter cloned in pGEM-T Easy)This study
    pPSV4pPSVΩ (SphI/SacI 574-bp fragment of cipC promoter cloned in pGEM-T Easy)This study
    pPSV5pPSVΩ (SphI/SacI 138-bp fragment of cipC promoter cloned in pGEM-T Easy)This study
    pPSV6pPSVΩ (SphI/SacI 250-bp fragment of cipC promoter cloned in pGEM-T Easy)This study
    pPSV7pPSVΩ (SphI/SacI 428-bp fragment of cipC promoter cloned in pGEM-T Easy)This study
    pPSV8pPSVΩ (SphI/SacI 452-bp fragment of cipC promoter cloned in pGEM-T Easy)This study
    pPSV9pPSVΩ (SphI/SacI 540-bp fragment of cipC promoter cloned in pGEM-T Easy)This study