TABLE 2.

Electron acceptor specificity of TcpBa

SubstrateSp actbRelative activity (%)
Menadione + FMN911.4 ± 64.5100
Ferricyanide + FMN179.1 ± 2.120
Menadione + FAD35.3 ± 0.64
Ferricyanide + FAD8.1 ± 0.62
FMN3.5 ± 0.50.4
FAD00
  • a Assays performed in 90 mM KPi buffer (pH 6.0) at 25°C. Values are means from three experiments with standard deviations.

  • b Specific activity is expressed as μmol NADH per minute per mg of protein.