TABLE 2.

Relative FtsZ levels in viable mre mutants

StrainaRelevant genotypeIPTG concn (μM)Relative FtsZ levelbSD
PA340wt01.0
PA340-129mreB12902.00.5
PA340-678mre-67802.40.8
PB103wt01.0
PB103/pCX16wt/sdiA04.21.6
FB2/pCX16mreB<>aph/sdiA03.00.7
FB2/pCH244mreB<>aph/Plac::mreBCD5001.00.2
FB2sup/pGB2mreB<>aph sup?c/vector02.6
  • a Cells were grown at 37°C in LB medium, supplemented with IPTG as indicated to an OD600 of 0.55 to 0.65, and prepared for quantitative Western analyses.

  • b Values were normalized to the level of FtsZ in the appropriate wt strain. Values given are averages for three measurements, except for FB2sup/pGB2, which was measured once.

  • c sup?, undefined suppressor.