TABLE 5.

Recovery of mreBCD<>aph and mrdAB<>aph transductants on minimal mediuma

P1 lysate and strainRelevant genotypeNo. of colonies for indicated temp (presence of IPTG)
20°C (−)30°C (−)30°C (+)37°C (−)37°C (+)
mreBCD<>aph
    PB103wtND103ND4ND
    PB103/pFB149wt/Plac::mreBCDND134149087
    TB28wt920NDNDND
mrdAB<>aph
    PB103wtND96ND6ND
    PB103(λTB59)wt(Plac::mrdAB)ND11298328
    TB28wt840NDNDND
  • a Equal aliquots of saturated cultures were mixed with equal aliquots of each transducing lysate, and the mixtures were plated on M9-maltose supplemented with 25 μg/ml Kan and either no (−) or 250 μM (+) IPTG. Plates were incubated at the indicated temperatures, and transductant colonies were counted. ND, not done.