TABLE 2.

Mutant strains constructed and/or used in this study

StrainKey plasmid usedisc gene(s) mutated in endogenous, IscR-controlled isc operona (Pisc)Gene(s) controlled by PscrXOther gene mutation(s)bGrowth on glucosec
DJNANonescrXNone+
DJ1411pDB1309NonescrX::kanNone+
DJ1418pDB1335NonelacZ-kanNone+
DJ1421pDB1316NoneiscSUA-hscBA-fdx-iscXNone+
DJ1434pDB1351ΔiscUAiscSUA-hscBA-fdx-iscXNone
DJ1445pDB1350ΔiscUiscSUA-hscBA-fdx-iscXrecA::kan
DJ1447pDB1291ΔhscBAiscSUA-hscBA-fdx-iscXrecA::kanSlow
DJ1448pDB1227iscU (IscUC106A)iscSUA-hscBA-fdx-iscXrecA::kan
DJ1450pDB954ΔiscSiscSUA-hscBA-fdx-iscXrecA::kan
DJ1451pDB1209iscS (IscSC325A)iscSUA-hscBA-fdx-iscXrecA::kan
DJ1452pDB1228iscU (IscUC63A)iscSUA-hscBA-fdx-iscXrecA::kan
DJ1453pDB1058iscU (IscUD39A)iscSUA-hscBA-fdx-iscXrecA::kan
DJ1454pDB1316NoneiscSUA-hscBA-fdx-iscXrecA::kan+
DJ1463pDB1016fdx::kaniscSUA-hscBA-fdx-iscXrecA::kan
DJ1476pDB1400NonelacZ-kanscrR::gn+
DJ1559pDB1391ΔiscAiscSUA-hscBA-fdx-iscXrecA::gn+
DJ1607pDB1538iscU (IscUH105A)iscSUA-hscBA-fdx-iscXrecA::kan
DJ1608pDB1546iscU (IscUK103A)iscSUA-hscBA-fdx-iscXrecA::kan
DJ1621pDB1550ΔfdxiscSUA-hscBA-fdx-iscXrecA::kan
DJ1656pDB1236iscA (IscAC36A)iscSUA-hscBA-fdx-iscXrecA::gn+
DJ1657pDB1215iscA (IscAC99A)iscSUA-hscBA-fdx-iscXrecA::gn+
DJ1661pDB1351ΔiscUAiscSUA-hscBA-fdx-iscXNone
DJ1662pDB1216iscA (IscAC101A)iscSUA-hscBA-fdx-iscXrecA::gn+
DJ1670pDB1391ΔiscAiscSUA-hscBA-fdx-iscXNone+
DJ1692pDB1608NonehscBA-fdx-iscXNone+
DJ1694pDB1291ΔhscBAhscBA-fdx-iscXrecA::kan
DJ1695pDB1550ΔfdxhscBA-fdx-iscXrecA::kan
DJ1702pDB1468ΔiscAiscSUA-hscBhscA′-lacZ)fdx-iscXNone+
DJ1714pDB1634ΔiscAiscANone+
  • a Site-directed substitutions of specific codons in isc genes are described as the amino acid substitution corresponding to the gene product.

  • b Gene mutations relevant to this study. Antiobiotic gene cartridges are gn, encoding gentamycin resistance (Gnr), and kan, encoding kanamycin resistance (Knr).

  • c Refers to growth on Burks minimal medium containing glucose as the sole carbon source at 30°C and 20% O2. Growth on medium containing sucrose under the same conditions was normal.