TABLE 1.

Yeast two-hybrid interactions of flagellin proteins (sorted by prey)

BaitaPreybBait countcPrey descriptiond
TP0870TP000510DNA gyrase, subunit A (gyrA) (Bacillus subtilis)
TP0870TP004613Hypothetical protein (Treponema pallidum)
TP0868TP005010Conserved hypothetical protein (Borrelia burgdorferi)
TP0792TP00648Hypothetical protein
TP0870TP006611Hypothetical protein
TP0792TP016013Prolyl-tRNA synthetase (proS) (Escherichia coli)
TP0870TP01741Hypothetical protein
TP0870(TP0258)90Conserved hypothetical protein (Borrelia burgdorferi)
TP0870(TP0288)94Spore coat polysaccharide biosynthesis protein (spsF)
TP0870TP039614Flagellar basal-body rod protein (flgB) (Treponema denticola)
TP0870(TP0455)57Conserved hypothetical protein (Borrelia burgdorferi)
TP0870(TP0563)78Hypothetical protein
TP0870TP06296Hypothetical protein
TP0792TP06583Transmembrane protein, putative (Bacillus subtilis)*
TP0868TP06583Transmembrane protein, putative (Bacillus subtilis)*
TP0870TP06583Transmembrane protein, putative (Bacillus subtilis)*
TP0868(TP0661)57Hypothetical protein
TP0870(TP0661)57Hypothetical protein
TP0870TP07022Conserved hypothetical protein (Borrelia burgdorferi)
TP0792(TP0764)87Conserved hypothetical protein (Borrelia burgdorferi)
TP0870(TP0764)87Conserved hypothetical protein (Borrelia burgdorferi)
TP0870(TP0788)63Hypothetical protein
TP0792(TP0832)41Hypothetical protein
TP0868(TP0832)41Hypothetical protein
TP0870(TP0832)41Hypothetical protein
TP0870(TP0870)59Flagellar filament 31-kDa core protein (flaB3)
TP0870TP08731Hypothetical protein
TP0870(TP0907)77Conserved hypothetical protein (Borrelia burgdorferi)
TP0870(TP0989)107P26 (Borrelia burgdorferi)
TP0870(TP0993)167Rare lipoprotein A, putative (Borrelia burgdorferi)
  • a Flagellin proteins tested as baits against the whole-genome prey library of T. pallidum. FlaB1 = TP0868, FlaB2 = TP792, FlaB3 = TP0870.

  • b Preys identified with more than one flagellin are marked in boldface. Preys not reliably identified due to unspecific binding (binding to more than 5% of the tested baits) are shown in parentheses.

  • c The bait count represents the number of unique baits a prey has been found with and thereby indicates nonspecific binding (numbers are based on 450 baits screened). For example, TP0005 has been found with 10 different baits, including TP0870.

  • d Species names given after the prey description indicate the source of annotation for these proteins. *, TP0658 is most likely not a membrane protein since it is fairly well soluble (the misleading annotation appears to stem from a hydrophobic sequence in the middle of the protein).