TABLE 1.

Bacterial strains and plasmids

Strain or plasmidRelevant genetic markers or characteristic(s)Source or referencea
Strains
    DH5αsupE44 ΔlacU169(φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relABethesda Research Laboratories
    W3110F λ− IN(rrnD-rrnE) rph-1CGSC
    AB1133thr-1 leuB6 (gpt-proA)66 hisG4 argE3 thi-1 rfbD1 lacY1 ara-14 galK2 xyl-5 mtl-1 mgl-1 rpsL31 kdgK51 supE44CGSC
    EC100F mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara leu)7697 galU galK λ rpsL nupGEpicenter
    EC100DAs EC100 but pir+ (dihydrofolate reductase)Epicenter
    KM32argE3 his-4 leuB6 proA2 thr-1 ara-14 galK2 lacY1 mtl-1 xyl-1 thi-1 rpsL-31 tsx-33 supE44 Δ(recC ptr recB recD)::Ptac-gam-bet-exo cat38
    PR4246As W3110 but yiaH::KAN-2 [PR4254 (P1) × W3110]This study
    PR4254As KM32 but yiaH::KAN-2This study
    PR4275EC100/pJun3This study
    PR4276As PR4275 but yiaH::KAN-2This study
    PR4279EC100D/pRL170This study
    PR4300PR4246/pRL180This study
Plasmids
    pGEM-T EasyPCR cloning vectorPromega
    pQE30Expression vectorQIAGEN
    pBR322Cloning vectorPromega
    pJun13.73-kb BamHI-HindIII fragment of pRL105b cloned into pBR322This study
    pJun3Wild-type wec gene cluster on a 12.78-kb EcoRI-BamHI fragment cloned into pBR322This study
    pRL170Rescue plasmid containing yiaH::KAN-2 mutant alleleThis study
    pRL171Wild-type yiaH gene on a 1.14-kb PCR fragment cloned into pGEM-T EasyThis study
    pRL172yiaH::KAN-2 on a 3.14-kb PCR fragment cloned into pGEM-T EasyThis study
    pRL179Wild-type yiaH on a 1.10-kb fragment cloned in pGEM-T EasyThis study
    pRL180Wild-type yiaH on a 1.10-kb BamHI-HindIII fragment cloned into pQE30This study
  • a CGSC, E. coli Genetic Stock Center and Mary Berlyn, Yale University, New Haven, Conn.

  • b See reference 34.