TABLE 2.

Viabilities of spores of various strainsa

Genotype (strain)Viability (%)b
Wild type (PS832)100
ΔgerQ::spc amyE::PgerQ 1-181 ΔsleB::tet (AM30)83
ΔgerQ::spc amyE::PgerQ 6-181 ΔsleB::tet (AM31)27
ΔgerQ::spc amyE::PgerQ 17-181 ΔsleB::tet (AM78)36
ΔgerQ::spc amyE::PgerQ 24-181 ΔsleB::tet (AM33)14
ΔgerQ::spc amyE::PgerQ 1-181 K2Q K4Q K5Q
    ΔsleB::tet (AM79)19
ΔgerQ::spc amyE::PgerQ 1-181 K2A K4A K5A
    ΔsleB::tet (AM80)72
ΔsleB::tet (FB110)122
ΔgerQ::spc ΔsleB::tet (KB30)0.03
ΔsleB::spc Δtgl::ermC (KB82)128
  • a Spores at an optical density at 600 nm of 1 were heat activated at 70°C for 30 min and cooled on ice, serial dilutions in water were spotted onto LB agar plates, the plates were incubated at 30°C and/or 37°C for 24 h, and colonies were counted as described in Materials and Methods.

  • b For each strain, the number of CFU per ml was determined. Wild-type spores had an average value of 1.8 × 108 CFU/ml, which was then set to 100%. Values shown are the averages of two independent experiments. The individual experimental values varied by an average of 19% from the average value.