TABLE 1.

Strains and plasmids used in this study

Strain or plasmidGenotypePhenotype/descriptionaSource or referenceb
B. subtilis strains
    PS832Wild typeLaboratory stock
    AM14ΔgerQ::spc amyE::gerQ 1-181Cmr SpcrpAM9→KB29
    AM15ΔgerQ::spc amyE::gerQ 24-181Cmr SpcrpAM10→KB29
    AM16ΔgerQ::spc amyE::gerQ 49-181Cmr SpcrpAM11→KB29
    AM17ΔgerQ::spc amyE::gerQ 61-181Cmr SpcrpAM12→KB29
    AM18ΔgerQ::spc amyE::gerQ 87-181Cmr SpcrpAM13→KB29
    AM23ΔgerQ::spc amyE::gerQ 6-181Cmr SpcrpAM21→KB29
    AM24ΔgerQ::spc amyE::gerQ 17-181Cmr SpcrpAM22→KB29
    AM30ΔgerQ::spc amyE::gerQ 1-181 ΔsleB::tetCmr Spcr TetrFB110→AM14
    AM31ΔgerQ::spc amyE::gerQ 6-181 ΔsleB::tetCmr Spcr TetrFB110→AM23
    AM33ΔgerQ::spc amyE::gerQ 24-181 ΔsleB::tetCmr Spcr TetrFB110→AM15
    AM45ΔgerQ::spc amyE::gerQ 1-181 K2QCmr SpcrpAM39→KB29
    AM46ΔgerQ::spc amyE::gerQ 1-181 K2ACmr SpcrpAM40→KB29
    AM47ΔgerQ::spc amyE::gerQ 1-181 K4QCmr SpcrpAM41→KB29
    AM48ΔgerQ::spc amyE::gerQ 1-181 K4ACmr SpcrpAM42→KB29
    AM49ΔgerQ::spc amyE::gerQ 1-181 K5QCmr SpcrpAM43→KB29
    AM50ΔgerQ::spc amyE::gerQ 1-181 K5ACmr SpcrpAM44→KB29
    AM57ΔgerQ::spc amyE::gerQ 1-181 K2Q,K4QCmr SpcrpAM54→KB29
    AM58ΔgerQ::spc amyE::gerQ 1-181 K2Q K5QCmr SpcrpAM55→KB29
    AM59ΔgerQ::spc amyE::gerQ 1-181 K4Q K5QCmr SpcrpAM56→KB29
    AM66ΔgerQ::spc amyE::gerQ 1-181 K2A K4ACmr SpcrpAM63→KB29
    AM67ΔgerQ::spc amyE::gerQ 1-181 K2A K5ACmr SpcrpAM64→KB29
    AM68ΔgerQ::spc amyE::gerQ 1-181 K2Q K4Q K5QCmr SpcrpAM65→KB29
    AM75ΔgerQ::spc amyE::gerQ 1-181 K4A K5ACmr SpcrpAM72→KB29
    AM76ΔgerQ::spc amyE::gerQ 1-181 K2A K4A K5ACmr SpcrpAM73→KB29
    AM78ΔgerQ::spc amyE::gerQ 17-181 ΔsleB::tetCmr Spcr TetrFB110→AM24
    AM79ΔgerQ::spc amyE::gerQ 1-181 K2Q K4Q K5Q ΔsleB::tetCmr Spcr TetrFB110→AM68
    AM80ΔgerQ::spc amyE::gerQ 1-181 K2A K4A K5A ΔsleB::tetCmr Spcr TetrFB110→AM76
    FB110ΔsleB::tetTetr17
    KB29ΔgerQ::spcSpcr18
    KB30ΔgerQ::spc ΔsleB::tetSpcr Tetr18
    KB82ΔsleB::spc Δtgl::ermCMLSr Spcr19
Plasmids
    pAM2PgerQgerQ promoter in pBluescript KS(−)This work
    pAM3PgerQ 1-181gerQ promoter + gerQ 1-181 in pBluescript KS(−)This work
    pAM9PgerQ 1-181gerQ promoter + gerQ 1-181 in pDG364This work
    pAM10PgerQ 24-181gerQ promoter + gerQ 24-181 in pDG364This work
    pAM11PgerQ 49-181gerQ promoter + gerQ 49-181 in pDG364This work
    pAM12PgerQ 61-181gerQ promoter + gerQ 61-181 in pDG364This work
    pAM13PgerQ 87-181gerQ promoter + gerQ 87-181 in pDG364This work
    pAM21PgerQ 6-181gerQ promoter + gerQ 6-181 in pDG364This work
    pAM22PgerQ 17-181gerQ promoter + gerQ 17-181 in pDG364This work
    pAM39PgerQ 1-181 K2QgerQ promoter + gerQ 1-181 K2Q in pDG364This work
    pAM40PgerQ 1-181 K2AgerQ promoter + gerQ 1-181 K2A in pDG364This work
    pAM41PgerQ 1-181 K4QgerQ promoter + gerQ 1-181 K4Q in pDG364This work
    pAM42PgerQ 1-181 K4AgerQ promoter + gerQ 1-181 K4A in pDG364This work
    pAM43PgerQ 1-181 K5QgerQ promoter + gerQ 1-181 K5Q in pDG364This work
    pAM44PgerQ 1-181 K5AgerQ promoter + gerQ 1-181 K5A in pDG364This work
    pAM54PgerQ 1-181 K2Q K4QgerQ promoter + gerQ 1-181 K2Q K4Q in pDG364This work
    pAM55PgerQ 1-181 K2Q K5QgerQ promoter + gerQ 1-181 K2Q K5Q in pDG364This work
    pAM56PgerQ 1-181 K4Q K5QgerQ promoter + gerQ 1-181 K4Q K5Q in pDG364This work
    pAM63PgerQ 1-181 K2A K4AgerQ promoter + gerQ 1-181 K2A K4A in pDG364This work
    pAM64PgerQ 1-181 K2A K5AgerQ promoter + gerQ 1-181 K2A K5A in pDG364This work
    pAM65PgerQ 1-181 K2Q K4Q K5QgerQ promoter + gerQ 1-181 K2Q K4Q K5Q in pDG364This work
    pAM72PgerQ 1-181 K4A K5AgerQ promoter + gerQ 1-181 K4A K5A in pDG364This work
    pAM73PgerQ 1-181 K2A K4A K5AgerQ promoter + gerQ 1-181 K2A K4A K5A in pDG364This work
    pBC KS(−)pBluescript KS(−)Stratagene
    pDG364amyE::catIntegrates at the amyE locus in the genome4
  • a Abbreviations: Cmr, chloramphenicol resistant; Spcr, spectinomycin resistant; Tetr, tetracycline resistant; MLSr, resistant to erythromycin and lincomycin.

  • b DNA from the strain or plasmid to the left of the arrow was used to transform the strain to the right of the arrow.