TABLE 1.

Oligonucleotides and primers used in this study

Oligonucleotide or primerSequence
SpeB-F5′-GGTGTCAGATTATTAAGTCTTTTAGC-3′
speB-R5′-CGAGAGCTACCTGCAGAAC-3′
prsA16245′-GCTCAAAAGGACATGAAAC-3′
prsA6915′-GGGCAGGTTGTTTTTGGTTAGG-3′
prsA15955′-ACTCATTACTGGTGTGGTAACGCTGG-3′
speB38055′-AGACGCTATGAGTCGTTGCACTTATC-3′
speB42785′-AGGCGCCTACTATGTGGTTATTGTTAC-3′
prsAext25′-GCTGCTCGTTGTGACTGTTCGTTAG-3′
prsAext35′-AGGATAGGTTAGGTTGTGGTG-3′
speBext15′-CTGCATTTGCTTTTAACGGTAC-3′
speBext25′-GTTTGTCAATGCTTTTATTGAC-3′
198BamHI5′-GGATCCACTCCAAAAAAGTTTTGGCTGATGAGCCTACTAGCTATCAG-3′
126XhoI5′-CCCCTCGAGATCAGGAGACACCAGAGCGCACAC-3′