TABLE 4.

Relative promoter strengths of various promoter collections in F. johnsoniae, F. hibernum, and E. coli

PromoterSequencea% GCRelative promoter activity in arbitrary fluorescence units (n-fold change)d
F. johnsoniaeF. hibernumE. coli
ConsensusNNNNNNTTGNNNNNNNNNNNNNNNNNNNTANNTTTGNNNNN
DL69TTAAGGTTGCGGGCGAGATGGTTTTATTTAGGTTTGGTTGA410 ± 0 (0)21 ± 1 (2.1)113 ± 4 (0.3)
DL15TGCTGGTTGTAGTGTCAGTAAATTTTGGTAAGTTTGTCCGG416 ± 1 (0.2)v1,248 ± 42 (124.8)265 ± 8 (0.8)
DL82GGGTTCTTGTCACGCGAGGTCATCATTTTATTTTTGTGCTT4140 ± 1 (1.2)12 ± 1 (1.2)475 ± 3 (1.5)
DL65TTGCGGTTGTCCTATTGTTGGTTACTGATACATTTGTTATC3781 ± 4 (2.4)29 ± 2 (2.9)542 ± 11 (1.7)
DL79TTTATGTTGTGACGTGTTGTCTCTGATTTAATTTTGGTGAA32100 ± 2 (3)190 ± 4 (19.0)257 ± 7 (0.8)
DL63TGCATGTTGTAGAGTCTCTGCATCTAAGTAAATTTGTAGGG39161 ± 2 (4.9)38 ± 2 (3.8)318 ± 7 (1)
DL05GGGGTATTGCTGTCTTAATAGACGGGTTTACCTTTGTACAG44172 ± 3 (5.1)51 ± 1 (5.1)329 ± 7 (1)
DL53TACGTTTTGGTTTTTTGATATAAGCCGATACTTTTGTATGT29323 ± 4 (9.6)73 ± 2 (7.3)490 ± 3 (1.5)
DL76TTGGTTTTGCTGGGTAATACATATTAAATACATTTGTAACT27875 ± 82 (26.1)557 ± 4 (55.7)334 ± 17 (1)
DL19GGCGAGTTGGTTGAACGTATAATTTTAGTATTTTTGGCCAG39881 ± 101 (26.2)808 ± 24 (80.8)499 ± 4 (1.5)
DL64TACGTTTTGTGCAAATTCTAATGATTTATATTTTTGTGGGT271,005 ± 31 (28.7)260 ± 4 (26.0)205 ± 4 (0.6)
DL52TCAGCGTTGTTATAGTGTTCAAGTTTTGTATTTTTGGTTGG34963 ± 31 (28.7)317 ± 3 (31.7)525 ± 6 (1.6)
DL58TTCGGTTTGTTGTGAATATTTTTCTTGGTAAATTTGTGTGA291,238 ± 13 (36.9)351 ± 1 (35.1)110 ± 1 (0.3)
DL04TTCGCTTTGATATTTAGTCTATGGTCGGTATCTTTGTGATC371,246 ± 19 (37.2)1,453 ± 39 (145.3)1,180 ± 26 (3.6)
DL97ACGTTGTTGTTAAGGGATGGTTTTTTTGTATGTTTGCCTGA371,309 ± 85 (39)403 ± 9 (40.3)541 ± 7 (1.7)
DL88TTATATTTGAGATTAGGTATAATCTTATTATTTTTGCTGTT201,726 ± 52 (51.5)479 ± 4 (47.9)352 ± 8 (1.1)
DL06CTTATTTTGCTTATTGTTTTCTAGTTAGTACTTTTGTTCGT27594 ± 0 (59.8)439 ± 4 (43.9)324 ± 2 (1)
DL16TAGATCTTGCTGTTTGGGATAGAATTAGTAAATTTGCGGGT372,371 ± 31 (70.7)1,016 ± 18 (101.6)519 ± 10 (1.6)
DL29GAATTTTTGTTTTTTGTCGTGAAATTTGTATATTTGTAGGT242,512 ± 33 (74.9)2,870 ± 91 (287.0)298 ± 12 (0.9)
DL84TACCTTTTGGATTCTGGCCGTTTTTGAGTATGTTTGTATTG372,515 ± 16 (75)1,167 ± 12 (116.7)354 ± 6 (1.1)
DL35TAGTTTTTGGAGTAACTAAAATAAGTAGTAGATTTGTGGAT272,710 ± 12 (80.8)1,320 ± 11 (132.0)287 ± 2 (0.9)
DL01TTTCGTTTGTGGGAGAAAAATGTCTTTGTATATTTGTATGG322,892 ± 103 (86)1,895 ± 36 (189.5)166 ± 7 (0.5)
DL21TTGATTTTGTATTTAAAAAATTTGGTGTTACTTTTGCTCTG244,515 ± 43 (134.6)3,221 ± 22 (322.1)353 ± 7 (1.1)
DL93ACGGGGTTGCGAAACTGATAAATTATTGTACCTTTGGAAGG415,085 ± 41 (151.6)2,190 ± 43 (219.0)803 ± 38 (2.5)
DL68TTTCCTTTGTATGTTTACTAAAAGTTTTTAAATTTGTTTGC225,475 ± 67 (163.2)2,869 ± 105 (286.9)683 ± 15 (2.1)
DL22TTGATTTTGTATTTAAAAAATTTGGTGTTACTTTTGCCTGT246,324 ± 162 (188.6)4,221 ± 91 (422.1)1,180 ± 13 (3.6)
DL98TTGATTTTGTATTTAAAAAATTTGGTGTTACTTTTGCTTGT229,727 ± 175 (290)6,030 ± 55 (603.0)1,527 ± 21 (4.7)
Negative controlbNAcNA34 ± 2 (1.0)10 ± 4 (1.0)327 ± 9 (1.0)
  • a The clones are ordered according to promoter strength in F. johnsoniae. Matches to the oligonucleotide consensus sequences −33 and −7 regions are in boldface.

  • b Strains carrying pSCH144 (promoterless gfp) were used as the negative control.

  • c NA, not applicable.

  • d The promoter activity was normalized to that of the promoter clones carrying pSCH144 (defined as 1.0), as shown in parentheses. Triplicate samples were used, and the standard deviations are shown.