ECACYC content of wild-type and mutant strains of E. coli K-12 determined by reverse-phase HPLC

StrainTotal amt of ECACYCa (nmol)Amt of each ECACYC speciesb (nmol) (no. of O-acetyl groups/molecule)
PR4275 (wild type)18.598.65 (0)
5.82 (1)
1.87 (2)
1.26 (3)
0.99 (4)
PR4276 (mutant)17.947.94 (0)
0 (1-4)
  • a Total amount of ECACYC in the soluble extracts obtained from cells grown in M9-glucose medium (50 ml) to an A600 of 1.0.

  • b ECACYC molecules synthesized by wild-type E. coli K-12 contain four trisaccharide repeat units substituted with from zero to four O-acetyl groups (15, 19). The numbers in parentheses indicate the number of O-acetyl groups/molecule.