TABLE 2.

Genes identified as being potentially involved in fimbrial biogenesis in D. nodosus

LocusaDesignationaNo. of amino acidsPotential functionRationaleb
DNO_0110fimA163Major pilin66% similarity to P. aeruginosa PilA/COG4969
DNO_0238fimX684Signal transduction protein59% similarity to P. aeruginosa FimX/COG4943
DNO_0344pilM337Fimbrial biogenesis60% similarity to P. aeruginosa PilM/COG4972
DNO_0345pilN215Fimbrial biogenesis59% similarity to P. aeruginosa PilN/COG3166
DNO_0346pilO222Fimbrial biogenesis61% similarity to P. aeruginosa PilO/COG3167
DNO_0347pilP206Fimbrial biogenesis57% similarity to P. aeruginosa PilP/COG3168
DNO_0348pilQ734Secretin67% similarity to P. aeruginosa PilQ/COG4796
DNO_0434pilS581Two-component system/sensor protein59% similarity to P. aeruginosa PilS/COG0642
DNO_0435pilR528Two-component system/response regulator70% similarity to P. aeruginosa PilR/COG2204
DNO_0515pilF253Fimbrial assembly60% similarity to P. aeruginosa PilZ/COG3063
DNO_0532rpoN469σ51 factor70% similarity to P. aeruginosa RpoN/COG1508
DNO_0675pilT357Twitching motility ATPase86% similarity to P. aeruginosa PilT/COG2805
DNO_0676pilU406Twitching motility ATPase76% similarity to P. aeruginosa PilU/COG5008
DNO_0712fimV959Fimbrial biogenesis53% similarity to P. aeruginosa FimV/COG3170
DNO_0890pilE151Pilin-like protein69% similarity to P. aeruginosa PilE/COG4968
DNO_0891fimU159Pilin-like protein58% similarity to P. aeruginosa FimU/T/COG4970
DNO_0892pilX221Pilin-like protein53% similarity to P. aeruginosa PilX/COG4726
DNO_0893pilW381Pilin-like protein46% similarity to P. aeruginosa PilW/COG4966
DNO_0894pilV189Pilin-like protein52% similarity to P. aeruginosa PilV/COG4967
DNO_0895pilC1272Fimbrial biogenesis/adhesin52% similarity to N. gonorrhoeae PilC/COG3419
DNO_1093chpA2554CheA/CheY hybrid, chemosensory55% similarity to P. aeruginosa ChpA/COG0643
DNO_1094pilJ436PilJ homolog, chemosensory54% similarity to P. aeruginosa PilJ/COG0840
DNO_1095pilI173CheW-like protein, chemosensory54% similarity to P. aeruginosa PilI/COG0835
DNO_1096pilH120CheY-like protein, chemosensory87% similarity to P. aeruginosa PilH/COG0745
DNO_1097pilG127CheY homolog, chemosensory82% similarity to P. aeruginosa PilG/COG0745
DNO_1106ppk722Polyphosphate kinase70% similarity to P. aeruginosa Ppk/COG0855
DNO_1124fimP286Prepilin peptidase77% similarity to P. aeruginosa PilD/XcpA/COG1989
DNO_1125fimO407Fimbrial assembly78% similarity to P. aeruginosa PilC/COG1459
DNO_1126fimN564Fimbrial assembly80% similarity to P. aeruginosa PilB/COG2804
DNO_1215pilZ120Fimbrial assembly76% similarity to P. aeruginosa PilZ/COG3215
  • a Locus names and designations are as previously described (30, 34, 54, 61).

  • b Percentage of overall similarity as determined by CLUSTAL W alignments.