TABLE 2.

Oligonucleotide primers used in this study

PrimerSequence (5′-3′)a
LmrANGGTGGGAATCATATGAGTTATG
LmrABbCGCGGATCCATGCTTTAGCTGTTGTTTCC
YxaFECCGGAATTCAGTCGCTCTACCATACCTAG
YxaFNGGGAATGTTCATATGACTAGCA
YxaFBCGCGGATCCTCCTCCAATTTAGACCAGTC
MK1bCAGTCAAAAGATCAGTCAGC
MK2bTGCCGTAATACCCTTGCAGC
PyxaG1bGGTGAGGAAAAAAGGGTAGC
PyxaG2bCTCCGGAGCAAATAAGGCAT
PyxaF1CTGTTGTTCAAGCTGCTCTG
PyxaF2TTCACGTGAATCTCCTCTGC
yxaFupFCTGCCGGTGTAGTGTTTGACAGC
yxaFupR_catdownGAGATAATGCCGACTGTACTGTGAATCTCCTCTGCTAGTCAT
yxaFdownF_catupCTAATGGGTGCTTTAGTTGAAGAACCAGTGCTGGTGAGGAAAAAAGGG
yxaFdownRCAATTGTCGCTACAGCGGCTGC
catFTCTTCAACTAAAGCACCCATTAG
catRAGTACAGTCGGCATTATCTC
yxaFupF2CGCTCTTGTTCCGTAAACAGGTCC
yxaFdownR2AAATCGCTCGTTTGAGACTTCTTCG
  • a Each restriction enzyme site is underlined.

  • b Primers are the same as those used in a previous study (30).